Optimization using Response Surface Methodology (RSM) for the formulation of a functional beverage based on whey, peach (Prunus persica) and bee pollen

Main Article Content

Liz Avelina Avalos Rozas
Liz Mery Mendieta Navio
Carlos Daniel Huachllanqui Olivera
Suimer Pareja Ramos

Abstract

This research project, submitted to the FERCYT competition in the scientific poster category, aims to develop an innovative functional beverage based on whey, peach (Prunus persica), and bee pollen, leveraging the high nutritional value and bioactive compounds of these ingredients. The objective is to optimize the product formulation by applying Response Surface Methodology (RSM), determining the ideal combination that maximizes nutritional and sensory properties. The research is applied and experimental, with an explanatory approach, focused on evaluating the influence of the proportions of whey, peach, and bee pollen on the beverage's physicochemical and sensory characteristics. A Box-Behnken design with three factors and fifteen experimental formulations was used. The response variables considered were pH, acidity, °Brix, and overall consumer acceptance, processed using Statgraphics XVII software, which allows for fitting second-order polynomial models and determining the optimal formulation conditions.

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How to Cite
Optimization using Response Surface Methodology (RSM) for the formulation of a functional beverage based on whey, peach (Prunus persica) and bee pollen. (2025). Micaela Revista De Investigación - UNAMBA, 6(2), 40-49. https://doi.org/10.57166/micaela.v6.n2.2025.186
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How to Cite

Optimization using Response Surface Methodology (RSM) for the formulation of a functional beverage based on whey, peach (Prunus persica) and bee pollen. (2025). Micaela Revista De Investigación - UNAMBA, 6(2), 40-49. https://doi.org/10.57166/micaela.v6.n2.2025.186

References

B. M. Popkin y C. Hawkes, «Sweetening of the global diet, particularly beverages: Patterns, trends, and policy responses», Lancet Diabetes Endocrinol, vol. 4, n.o 2, pp. 174-186, feb. 2016, doi: 10.1016/S2213-8587(15)00419-2.

I. N. de E. e I. INEI, «Perú: Encuesta Demográfica y de Salud Familiar 2024 - Nacional y Departamental», 2024. [En línea]. Dis-ponible en: https://www.gob.pe/institucion/inei/informes-publicaciones/6813623-peru-encuesta-demografica-y-de-salud-familiar-endes-2024

M. Lizárraga-Chaidez et al., «El inocente impacto ambiental del suero de la leche», Epistemus (Sonora), vol. 17, n.o 35, pp. 88-97, 2023, doi: 10.36790/EPISTEMUS.V17I35.316.

J. C. León Carrasco, «Producción nacional de quesos alcanzó las 145.765 toneladas en 2022, mostrando un incremento de 15% frente al año previo», Agencia Agraria de Noticias, 28 de marzo de 2023. Accedido: 14 de octubre de 2025. [En línea]. Disponible en: https://agraria.pe/noticias/produccion-nacional-de-quesos-alcanzo-las-145-765-toneladas--31289

C. Alvarado Carrasco y M. Guerra, «Lactosuero como fuente de péptidos bioactivos», Anales Venezolanos de Nutrición, vol. 23, n.o 1, pp. 45-50, 2010, Accedido: 29 de octubre de 2025. [En línea]. Disponible en: http://ve.scielo.org/scielo.php?script=sci_arttext&pid=S0798-07522010000100007&lng=es&nrm=iso&tlng=es

B. Denisow y M. Denisow-Pietrzyk, «Biological and therapeutic properties of bee pollen: a review», J Sci Food Agric, vol. 96, n.o 13, pp. 4303-4309, oct. 2016, doi: 10.1002/JSFA.7729;WGROUP:STRING:PUBLICATION.

C. A. Moreno Mena, «COMPARACIÓN NUTRICIONAL DEL SNACK DE FRUTA DESHIDRATADA DE NARANJA (Citrus X sinensis) Y DURAZNO (Prunus pérsica)», 2022.

E. K. Contreras Lujan, «OPTIMIZACIÓN DE LA BEBIDA FUNCIONAL A PARTIR DE AGUAYMANTO (Physalis peru-viana) Y MASHUA AMARILLA (Tropaeolum tuberosum) UTILIZANDO EL MÉTODO DE SUPERFICIE DE RESPUESTA», UNIVERSIDAD NACIONAL DE HUANCAVELICA, 2019.

R. S. Agrawal, R. C. Ranveer, N. B. Rathod, y N. P. Nirmal, «Phytochemicals as bioactive ingredients for functional foods», Recent Frontiers of Phytochemicals: Applications in Food, Pharmacy, Cosmetics, and Biotechnology, pp. 95-108, ene. 2023, doi: 10.1016/B978-0-443-19143-5.00028-1.

L. Fuentes Berrio, D. Acevedo Correa, C. A. Chantré, y V. M. Gelvez Ordoñez, «ALIMENTOS FUNCIONALES: IMPACTO Y RETOS PARA EL DESARROLLO Y BIENESTAR DE LA SOCIEDAD COLOMBIANA», Biotecnología en el Sector Ag-ropecuario y Agroindustrial, vol. 13, n.o 2, pp. 140-149, 2015, doi: 10.18684/BSAA(13)140-149.

Á. P. Aponte Colmenares et al., «Review. Aplicaciones del lactosuero y sus derivados proteínicos», Ciencia en Desarrollo, vol. 14, n.o 2, pp. 139-155, jul. 2023, doi: 10.19053/01217488.V14.N2.2023.15002.

J. Aguayo-Rojas et al., «Fitoquímicos y propiedades nutracéuticas de durazno (Prunus persica L.) cultivado en Zacatecas», Polibotanica, vol. 0, n.o 53, pp. 151-166, ene. 2022, doi: 10.18387/POLIBOTANICA.53.10.

D. J. Pérez Villarroel, «Obtención de una bebida fermentada alcohólica a base de mosto de durazno abridor (Prunus persica)», 16 de julio de 2019, Escuela Superior Politécnica de Chimborazo. Accedido: 29 de octubre de 2025. [En línea]. Disponible en: https://dspace.espoch.edu.ec/handle/123456789/11842

T. H. Roulston y J. H. Cane, «Pollen nutritional content and digestibility for animals», Plant Systematics and Evolution, vol. 222, n.o 1-4, pp. 187-209, 2000, doi: 10.1007/BF00984102.

E. W. HERBERT y H. SHIMANUKI, «CHEMICAL COMPOSITION AND NUTRITIVE VALUE OF BEE-COLLECTED AND BEE-STORED POLLEN», Apidologie, vol. 9, n.o 1, pp. 33-40, 1978, doi: 10.1051/APIDO:19780103.

M. Reji y R. Kumar, «Response surface methodology (RSM): An overview to analyze multivariate data», Indian Journal of Mi-crobiology Research, vol. 9, n.o 4, pp. 241-248, 2022, doi: 10.18231/J.IJMR.2022.042.

A. Czyrski y H. Jarzebski, «Response Surface Methodology as a Useful Tool for Evaluation of the Recovery of the Fluoroquin-olones from Plasma—The Study on Applicability of Box-Behnken Design, Central Composite Design and Doehlert Design», Processes 2020, Vol. 8, Page 473, vol. 8, n.o 4, p. 473, abr. 2020, doi: 10.3390/PR8040473.

D. Galvan, L. Effting, H. Cremasco, y C. A. Conte-Junior, «Recent applications of mixture designs in beverages, foods, and pharmaceutical health: A systematic review and meta-analysis», Foods, vol. 10, n.o 8, p. 1941, ago. 2021, doi: 10.3390/FOODS10081941/S1.

M. Wanyonyi, J. W. Kiluyi, y A. A. Okango, «Optimization of a Functional Beverage Formulation Using a Constrained Mixture Design with Multiple Responses», Asian Journal of Probability and Statistics, vol. 27, n.o 1, pp. 1-16, ene. 2025, doi: 10.9734/AJPAS/2025/V27I1699.

A. K. Jha y N. Sit, «Comparison of response surface methodology (RSM) and artificial neural network (ANN) modelling for supercritical fluid extraction of phytochemicals from Terminalia chebula pulp and optimization using RSM coupled with desirabil-ity function (DF) and geneti…», Ind Crops Prod, vol. 170, p. 113769, oct. 2021, doi: 10.1016/J.INDCROP.2021.113769.

M. A. Bezerra, R. E. Santelli, E. P. Oliveira, L. S. Villar, y L. A. Escaleira, «Response surface methodology (RSM) as a tool for optimization in analytical chemistry», Talanta, vol. 76, n.o 5, pp. 965-977, sep. 2008, doi: 10.1016/J.TALANTA.2008.05.019.

D. C. Montgomery, Design and Analysis of Experiments, John Wiley & Sons, vol. 106, n.o 11. 2017. [En línea]. Disponible en: https://www.researchgate.net/publication/362079778_Design_and_Analysis_of_Experiments_9th_Edition

J. Sun, T. Yang, Q. Liu, y Z. Sun, «Optimization of key technology for instant sea cucumber processing through fuzzy evaluation and response surface methodology», Am J Biochem Biotechnol, vol. 13, n.o 2, pp. 99-110, 2017, doi: 10.3844/AJBBSP.2017.99.110.

A. K. Jha y N. Sit, «Comparison of response surface methodology (RSM) and artificial neural network (ANN) modelling for supercritical fluid extraction of phytochemicals from Terminalia chebula pulp and optimization using RSM coupled with desirabil-ity function (DF) and geneti…», Ind Crops Prod, vol. 170, oct. 2021, doi: 10.1016/J.INDCROP.2021.113769.

H. Gutiérrez Pulido y R. de la Vara Salazar, Análisis y diseño de experimentos, 3.a ed. 2012. [En línea]. Disponible en: https://www.facebook.com/pages/Interfase