Diff-Quik and Eosin-Nigrosin dyes in the morphological evaluation of sperm before and after cryopreservation of Holstein bull semen
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Abstract
The objective of this study was to evaluate sperm morphological abnormalities using Diff-Quik (DQ) and Eosin-Nigrosin (EN) dyes before and after cryopreservation of bull semen. Four treatment (T) groups were formed T1: DQ staining before freezing; T2: EN staining before freezing, T3: DQ staining after freeze-thaw; T4: EN staining after semen freeze-thaw. Semen samples were collected from a 2-year-old Holstein bull, using an artificial vagina, twice a week for up to 10 collections. Fresh and after freeze-thaw semen was evaluated. Analysis of variance was calculated. Before and after freezing bovine semen, morphological abnormalities of the head and most of the neck and tail of the sperm using DQ or EN staining are similarly observed (P> 0.05). A greater (P ≤ 0.05) defect of proximal cytoplasmic gout and bent tail was identified in spermatozoa stained with EN than DQ in fresh and frozen bull semen. In conclusion, greater abnormalities of proximal cytoplasmic gout and bent tail of sperm are observed with EN staining than with DQ, while in the identification of the other anomalies there is no difference, before and after cryopreservation of semen from the Holstein bull
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