In vitro fertilization of alpaca oocytes, post-maturation supplemented with follicle-stimulating hormone and equine chorionic gonadotropin
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Abstract
El objetivo de este estudio fue evaluar los ovocitos de alpaca madurados in vitro, con suplemento de diferentes concentraciones de hormona folículo estimulante (FSH) y gonadotropina coriónica equina (eCG) y determinar
la segmentación embrionaria, post fertilización in vitro. Se recuperaron ovocitos de ovarios de alpacas sacrificadas, los
de categoría A y B se maduraron in vitro durante 36 h en medio TCM-199, suplementado con hormonas distribuidos
en tratamientos (T), siendo T1: FSH (0.05 UI mL-1) + eCG (40 UI mL-1) y T2: FSH (0.1 UI mL-1) + eCG (40 UI mL-1), luego fertilizados y cultivados in vitro hasta dos días. La cantidad de ovocitos en metafase II fue similar (P > 0.05)
entre T1 y T2 (62.9 ± 6.8 vs 66.3 ± 5.7%). La tasa de segmentación embrionaria fue similar (P > 0.05) entre T1 y T2
(47.4 ± 4.9 vs 53.9 ± 8.3%). En conclusión, la suplementación de FSH más eCG, en las concentraciones de este estudio,
presentan similares tasas de maduración de ovocitos in vitro y segmentación embrionaria en alpacas.
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